Confocal Microscope

Embryoid body with cell nuclei labeled with DAPI (blue), endothelial precursors labeled with CD31-Alexa 555 (red) and early hematopoietic progenitors labeled with CD34-Alexa 488 (green).

This is a Zeiss Axioskop 2 upright microscope with a Biorad 2000 laser head with several objective options, including a Zeiss 20x Plan Apochromat (N.A. 0.6), a Zeiss Plan Neofluar 40x oil phase (N.A. 1.3), the world class Zeiss 63x oil Apochromat (N.A. 1.4), and a Zeiss Plan Neofluar 100x oil phase (N.A. 1.3).

The scope has 3 lasers and is capable of excitation in a number of wavelengths:

1. tunable 15 mW argon laser can excite at 514, 488, 476, and 457 nm;
2. 10mW He/Ne that excites at 543nm
3. Red 637 nm for APC. 

The PMTs are selectable with the appropriate mirror and band pass filter. Experiments can use all three PMTs in a single pass.

This instrument has the capability of the standard FITC, PE, Texas Red, PerCP, Cy2, Cy3, ToPro3, APC, and numerous other fluorophores (click here for detailed specifications of filters and mirrors).  Consultations on applications are available. 

Current rate is $50/hr 

Training is required and is available by appointment.

For information, scheduling, or training please contact:

Genya Gekker
612-624-0441
gekke001@umn.edu
Office Hours: 9:00 a.m. – 5:00 p.m.