Karen Echeverri, PhD

Assistant Professor, Department of Genetics, Cell Biology and Development

Karen Echeverri

Contact Info

echev020@umn.edu

Office Phone 612-626-7320

Office Address:
Stem Cell Institute
4-222 LRB/MTRF
2001 6th St SE
Minneapolis, MN 55455

Mailing Address:
Stem Cell Institute
LRB/MTRF
2873B (Campus Delivery Code)
2001 6th St SE
Minneapolis, MN 55455

PhD Trinity College - Dublin and Max Planck Institute, 2003

BSc, Hons University College, 1996

Assistant Professor, Stem Cell Institute and Dept. Genetics, Cell Biology and Development, University of Minnesota, 2012

Independent Research Associate, Center for Regenerative Therapies, Dresden, Germany 2006-2011

Postdoc, Max Planck Institute, Molecular Cell Biology and Genetics 2004-2006

Research

Research Summary/Interests

Throughout human life, many cells such as hair follicles and certain tissues such as liver can be continuously replaced to maintain tissue integrity in response to normal, daily wear and tear. However, the human response to more serious tissue damage, such as acute damage to limbs or to the spinal cord, is limited to relatively simple wound healing, whereby collagenous scar tissue fills the injury site, assuring the tissue’s structural integrity but often resulting in a debilitating loss of functional activity. While humans do exhibit some very limited regenerative capacity (e.g. finger tips), other vertebrates exhibit sometimes astonishing regenerative ability.

Salamanders show the highest diversity in being able to regenerate limbs, tail, heart, eyes and jaw.

Our aim is to understand at the molecular and cellular level how an axolotl spinal cord can functionally repair after injury and why mammals cannot. To this end we have used transcriptional profiling to identify key differences at the miRNA level between axolotl and rat after spinal cord injury. In particular we are focusing on the differences between the rostral and caudal sides of the injury site and how the axolotl creates a permissive environment for axonal regrowth while mammals do not. We are focusing not just on the neuronal cells but also on the contribution and interaction of the other cells especially endothelial cells and skin to this repair process.

In addition to understanding spinal cord repair we are also interested in elucidating conserved pathways used for all regenerative processes like limbs, tails etc as well as those which tell cells at a cut surface what needs to be regenerated. 

Publications

  • Sehm T, Sachse, C , Frenzel, C and Echeverri K. miR-196 is an essential early-stage regulator of tail regeneration, upstream of key spinal cord patterning events. Dev Biol. 2009 Oct 15;334(2):468-80. Epub 2009 Aug 13.
  • Echeverri, K and AC Oates. 2007. Suppressor of Hairless coordinates bilateral cyclic gene expression during somitogenesis and plays an important role in left-right patterning of the vertebrate embryo. Dev. Biol. Jan 15;301(2):388-403.
  • Echeverri, K and EM Tanaka. 2005. Proximodistal Patterning during Limb Regeneration. Dev. Biol.279(2):391-401.
  • Echeverri, K. and EM Tanaka. 2002. Ectoderm to Mesoderm Lineage Switching during Axolotl Tail Regeneration. Science 298: 1993-1996
  • Echeverri K, Tanaka EM. Mechanisms of muscle dedifferentiation during regeneration. Semin Cell Dev Biol. 2002 Oct;13(5):353-60. Review.
  • Echeverri, K., Clarke, JD and EM Tanaka. 2001. In vivo imaging indicates muscle fiber dedifferentiation is a major contributor to the regenerating tail blastema. Dev. Biol. 236 (1):151-64.