Michael Kyba, PhD

Professor and Lillehei Endowed Scholar
Carrie Ramey / CCRF Endowed Professor in Pediatric Cancer Research, Department of Pediatrics

Michael Kyba

Contact Info

kyba@umn.edu

Office Phone 612-625-2173

Fax 612-624-8118

Office Address:
Pediatric BMT
Cancer & Cardiovascular Research Building
Lillehei Heart Institute
4-127 CCRB
2231 6th St SE
Minneapolis, MN 55455

Mailing Address:
Cancer & Cardiovascular Research Building
Lillehei Heart Institute
1st floor Mailroom CCRB
2231 6th St SE
Minneapolis, MN 55455

Professor and Lillehei Endowed Scholar
Carrie Ramey / CCRF Endowed Professor in Pediatric Cancer Research
, Department of Pediatrics

Pediatric Blood and Marrow Transplant (BMT) Center

Lillehei Heart Institute

Stem Cell Institute


Postdoctoral Fellowship, Massachusetts Institute of Technology - Whitehead Institute (Stem Cell Biology), 2003

PhD, University of British Columbia (Zoology), 1998

Summary

Dr. Kyba is a Professor of Pediatrics in the Division of Blood and Marrow Transplantation. He is also a member of the Lillehei Heart Institute, and an affiliate member of the Stem Cell Institute.

Dr. Kyba received his PhD degree from the University of British Columbia in 1998, and completed a postdoctoral fellowship in stem cell biology at the Whitehead Institute at MIT, Cambridge, MA in 2003. From 2003-2008, he was Assistant Professor of Developmental Biology at the University of Texas Southwestern Medical Center at Dallas, TX. He joined the faculty at the University of Minnesota in July 2008.

Dr. Kyba has published 35 research manuscripts in scientific journals, including: Cell, Science, and Nature Medicine.

Awards & Recognition

  • Dr. Marvin and Hadassah Bacaner Research Award, Minnesota Medical Foundation, 2010
  • Basil O’Connor Starter Scholar Research Award, March of Dimes, 2006
  • Virginia Murchison Linthicum Scholar in Biomedical Research, UT SW Medical Center at Dallas, 2003
  • Keystone Symposium Scholarship (X3-Stem Cells: Origins, Fates, Functions), 2002
  • Medical Research Council of Canada Postdoctoral Fellowship, 2000-2002

Research

Research Summary/Interests

Dr. Kyba’s research laboratory focuses on regulation of tissue-specific stem cells (hematopoietic and skeletal muscle) with a view towards ex-vivo expansion and therapeutic transplantation, as well as the derivation of tissue-specific stem cells from embryonic or iPS cells. He is also developing methods of performing BMT without irradiation or chemical conditioning. He has performed seminal experiments establishing the proof of principle for hematopoietic stem cell repopulation using embryonic stem cells and maintains an active program in the development of gene-targeting / genetic correction / cell therapy models.

Deriving therapeutic hematopoietic stem cells from embryonic stem cells.

ES cells are totipotent and capable of recapitulating all of the developmental events of embryogenesis. They are therefore theoretically the ideal source of cells for regenerative therapies. However, turning theory into practice is not straightforward, and very few successful models of such therapy exist. We have developed one successful model, based on regulated expression of members of the Hox family of transcription factors. Current work is focused on understanding how Hox genes regulate hematopoietic stem cell self-renewal and identifying regulatory circuits under Hox control.

Skeletal muscle stem cells and FSH muscular dystrophy

Certain degenerative diseases may be the result of ineffective self-renewal or differentiation of lineage specific stem cells. We are particularly interested in Fascioscapulohumeral Muscular Dystrophy (FSHD), a dominant dystrophy associated with a contraction of 4q subtelomeric repeats. Although the condition is almost certainly caused by derepression of a gene in the vicinity of 4q, the protein products of candidate genes in this area can not be detected overexpressed in patient muscle samples. Because muscle stem cells (satellite cells) are rare, proteins overexpressed specifically in satellite cells are unlikely to be identified in patient biopsies. We are testing the hypothesis that a Hox gene embedded within the 4q repeats, DUX4, causes FSHD when derepressed in muscle satellite cells.

Publications

A selection of Dr. Kyba’s Recent Publications

  • HoxA3 is an apical regulator of haemogenic endothelium. Iacovino M, Chong D, Szatmari I, Hartweck L, Rux D, Caprioli A, Cleaver O, Kyba M. Nat Cell Biol. 2011 Jan;13(1):72-8. doi: 10.1038/ncb2137. Epub 2010 Dec 19.
  • A new immuno- dystrophin-deficient model, the NSG-mdx4Cv mouse, provides evidence for functional improvement following allogeneic satellite cell transplantation. Arpke RW, Darabi R, Mader TL, Zhang Y, Toyama A, Lonetree C, Nash N, Lowe DA, Rita C.R. Perlingeiro, Kyba M. Stem Cells. 2013 31:1611-1620. doi: 10.1002/stem.1402.
  • A focal domain of extreme demethylation within D4Z4 in FSHD2. Hartweck LM, Anderson LJ, Lemmers RJ, Dandapat A, Toso EA, Dalton JC, Tawil R, Day JW, van der Maarel SM, Kyba M. Neurology. 2013 80:392-299. doi: 10.1212/WNL.0b013e31827f075c. Epub 2013 Jan 2.
  • Mesp1 patterns mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner. Chan SS, Shi X, Toyama A, Arpke RW, Dandapat A, Iacovino M, Kang JJ, Le G, Hagen HR, Garry DJ, Kyba M. Cell Stem Cell. 2013 12:587-601. doi: 10.1016/j.stem.2013.03.004.
  • High-throughput screening identifies inhibitors of DUX4-induced myoblast toxicity. Bosnakovski D, Choi SH, Strasser JM, Toso EA, Walters MA, Kyba M. Skeletal Muscle. 2014 (1):4. doi: 10.1186/2044-5040-4-4.

Click here to see a complete list of Dr. Kyba’s studies which have been published by academic and research journals.