Naoko Koyano, PhD, MS

Associate Professor of Medicine, Cardiovascular Division

Naoko Koyano

Contact Info

koyano@umn.edu

Mailing Address:
Cancer and Cardiovascular Research Building
2231 6th St SE
4-145
Minneapolis, MN

Lab Address:
Cancer and Cardiovascular Research Building
2231 6th St SE
4-210
Minneapolis, MN

Administrative Assistant Name
Shawn Jarvis

Administrative Phone
612-625-9538

Administrative Email
jarvi178@umn.edu

Associate Professor of Medicine, Cardiovascular Division

Cardiology

Member, Stem Cell Institute

Lillehei Heart Institute


PhD, Biochemistry and Biophysics, University of Tokyo, Tokyo, Japan

MS, Biochemistry and Biophysics, University of Tokyo, Tokyo, Japan

BSc, Biochemistry and Biophysics, University of Tokyo, Tokyo, Japan

Postdoctoral Fellow, Salk Institute for Biological Studies

Summary

Dr. Koyano-Nakagawa is an Associate Professor at the Lillehei Heart Institute and a member of the Stem Cell Institute. She received her B.Sc., M.Sc. and Ph.D. from the University of Tokyo, Japan, studying cytokine gene regulation under the direction of Drs. Ken-ichi Arai and Takashi Yokota. She worked with Dr. Chris Kintner as a postdoctoral fellow at the Salk Institute for Biological Studies and investigated functions and regulatory mechanisms of neuronal transcription factors. She currently works on molecular mechanisms controlling development and regeneration of the heart and mesodermal derivatives.

Expertise

  • developmental biology
  • embryology
  • molecular biology
  • transcriptional regulation

Awards & Recognition

  • Bacaner Research Award in Cardiology (2015)
  • Japan Overseas Educational Services, Educational Grant (2013)
  • Postdoctoral Fellowship, Uehara Memorial Foundation (1999-2000)
  • Long-Term Fellowship, Human Frontier Science Program (1997-1999)
  • Postdoctoral Fellowship, Uehara Memorial Foundation (1997-1998)

Research

Research Summary/Interests

Our research interest is to understand the mechanisms regulating normal development of the cardiovascular system and to explore the regenerative capacity of the mammalian heart, which is a poorly regenerative organ. We believe that understanding of basic biological mechanisms will ultimately lead to development of methods to repair an injured heart. We utilize mouse genetic models, ES and iPS cells to address such questions.

Research Funding Grants

  1. Humanized vasculature in gene edited animals, Regenerative Medicine Minnesota, (Daniel J. Garry, program PI), Naoko Koyano-Nakagawa, Co-I, $82,237, 04/01/16 – 03/30/18 (direct costs, annual project period) 1% effort
  2. Exogenic Human Heart in Gene-Edited Animals, GRANT11763537, Department of Defense (DoD), (Daniel J. Garry, program PI), Naoko Koyano-Nakagawa, Co-I, $960,000, 07/01/15 – 06/30/18 (direct costs, annual project period) 10% effort
  3. Regulatory Mechanisms of Endothelial Development and Regeneration, 1R01HL122576-01, National Institutes of Health (NIH)/National Heart, Lung, and Blood Institute (NHLBI), (Daniel J. Garry, program PI), Naoko Koyano-Nakagawa, Co-I, $250,000, 04/01/14 – 03/31/19 (direct costs, annual project period) 10% effort

Publications

  • Baik J, Magli A, Swanson SA, Borges L, Koyano-Nakagawa N, Stewart R, Garry DJ, Thomson JA, Perlingeiro RCR. Endoglin integrates BMP and Wnt signaling to induce hematopoiesis through JDP2. Nature Communications, 2016; 7:13101-. PMID 27713415
  • Persaud SD, Park SW, Ishigami-Yuasa, M, Koyano-Nakagawa N, Kagechika H, Wei L-N. All trans-retinoic acid analogs promote cancer cell apoptosis through non-genomic Crabp1 mediating ERK1/2 phosphorylation. Scientific Reports, 2016; 6:22396-. PMCID: PMC4776112
  • Singh BN, Kawakami Y, Akiyama R, Rasmussen TL, Garry MG, Gong W, Das S, Shi X, Koyano-Nakagawa, N, Garry DJ. Etv2-miR-130a network regulates mesodermal specification. Cell Reports, 2015; 13(5):915-23. PMCID: PMC4747240
  • Koyano-Nakagawa N, Shi X, Rasmussen TL, Walter CA, Garry DJ. Feedback mechanisms regulate Ets variant 2 (Etv2) gene expression and hematoendothelial lineages. J Biol. Chem,, 2015: 290(47):28107-19. PMID: 26396195. Featured as the cover image. PMCID: PMC4653670
  • Shi X, Zirbes KM, Rasmussen TL, Ferdous A, Garry MG, Koyano-Nakagawa N, Garry DJ. The transcription factor, Mesp1, interacts with cAMP responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression. Journal of Biological Chemistry. 2015; 290(15): 9614-25. PMCID: PMC4392264
  • Gong WM, Koyano-Nakagawa N, Li TB, Garry DJ. Inferring dynamic gene regulatory networks in cardiac differentiation through the integration of multi-dimensional data. BMC Bioinformatics. 2015; 2015;16(1):74. PMCID: PMC4359553
  • Shi X, Richard J, Zirbes KM, Gong W, Lin G, Kyba M, Thomson JA, Koyano-Nakagawa N, Garry DJ. Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages. Developmental Biology. 2014;389(2):208-218. PMCID: PMC4099474
  • Rasmussen TL, Martin CM, Walter CA, Shi X, Perlingeiro R, Koyano-Nakagawa N, Garry DJ. Etv2 rescues Flk1 mutant embryoid bodies. Genesis. 2013;51(7):471-480. PMCID: PMC3987665
  • Borges L, Iacovino M, Koyano-Nakagawa N, Baik J, Garry DJ, Kyba M, Perlingeiro RC. Expression levels of endoglin distinctively identify hematopoietic and endothelial progeny at different stages of yolk sac hematopoiesis. Stem Cells. 2013;31(9):1893-1901. PMCID: PMC3795927
  • Singh BN, Doyle MJ, Weaver CV, Koyano-Nakagawa N, Garry DJ. Hedgehog and Wnt coordinate signaling in myogenic progenitors and regulate limb regeneration. Developmental Biology. 2012;371(1):23-34. Featured as the cover image. PMCID: PMC3987681
  • Rasmussen TL, Shi X, Wallis A, Kweon J, Zirbes KM, Koyano-Nakagawa N, Garry DJ. VEGF/Flk1 signaling cascade transactivates Etv2 gene expression. PLoS One. 2012;7(11):e50103. PMCID: PMC3501484
  • Koyano-Nakagawa N, Kweon J, Iacovino M, Shi X, Rasmussen TL, Borges L, Zirbes KM, Li T, Perlingeiro RC, Kyba M, Garry DJ. Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression. Stem Cells. 2012;30(8):1611-1623. PMCID: PMC3651838
  • Borges L, Iacovino M, Mayerhofer T, Koyano-Nakagawa N, Baik J, Garry DJ, Kyba M, Letarte M, Perlingeiro RC. A critical role for endoglin in the emergence of blood during embryonic development. Blood. 2012;119(23):5417-5428. PMCID: PMC3369678
  • Rasmussen TL, Kweon J, Diekmann MA, Belema-Bedada F, Song Q, Bowlin K, Shi X, Ferdous A, Li T, Kyba M, Metzger JM, Koyano-Nakagawa N, Garry DJ. ER71 directs mesodermal fate decisions during embryogenesis. Development. 2011;138(21):4801-4812. PMCID: PMC3190388
  • Caprioli A*, Koyano-Nakagawa N*+, Iacovino M*, Shi X, Ferdous A, Harvey RP, Olson EN, Kyba M, Garry DJ+. Nkx2-5 represses Gata1 gene expression and modulates the cellular fate of cardiac progenitors during embryogenesis. Circulation. 2011; 123(15):1633-1641. [*Co-first author; +Co-corresponding author] PMCID: PMC3110259